col4a2 polyclonal antibody  (Proteintech)

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and COL4A2 gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemistry, Western Blot, Control, Functional Assay, RNA Sequencing

Journal: Frontiers in Immunology

Article Title: Integration of single-cell sequencing and machine learning identifies key macrophage-associated genetic signatures in lumbar disc degeneration

doi: 10.3389/fimmu.2025.1671961

Figure Lengend Snippet: Immune infiltration analysis. (A) Quantitative analysis of immune cells; (B) Immune infiltration characteristics among samples; (C) Cell interaction network analysis; (D) Gene–immune correlation analysis; (E–G) Western blot detection of CDK1 and COL4A2 expression levels in LDD rat model at 1, 2, and 3 months post-surgery; (H, I) qPCR detection of CDK1 and COL4A2 mRNA levels at 1, 2, and 3 months post-surgery. Data are presented as mean ± SD, with experiments repeated three times; *P < 0.05, **P < 0.01, ***P < 0.001, **** indicates that there is a highly significant correlation between the two (one-way ANOVA with Bonferroni post hoc test).

Article Snippet: Antibodies: COL4A2 (Collagen Type IV Alpha 2 Chain, 1:1000) – Proteintech (#14695-1-AP, RRID: AB_10699877); CDK1 (Cyclin-Dependent Kinase 1, 1:1000) – Proteintech (#19532-1-AP, RRID: AB_2881388); MMP3 (Matrix Metalloproteinase 3, 1:1000) – Proteintech (#17873-1-AP, RRID: AB_2138307); SRGN (Serglycin, 1:1000) – Thermo Fisher Scientific (#PA5-113692, RRID: AB_2884207); HRP-conjugated anti-mouse IgG (H+L, 1:5000) – Proteintech (#SA00001-1, RRID: AB_2722565); HRP-conjugated anti-rabbit IgG (H+L, 1:5000) – Proteintech (#SA00001-2, RRID: AB_2722564).

Techniques: Western Blot, Expressing

Journal: Frontiers in Immunology

Article Title: Integration of single-cell sequencing and machine learning identifies key macrophage-associated genetic signatures in lumbar disc degeneration

doi: 10.3389/fimmu.2025.1671961

Figure Lengend Snippet: Screening of potential therapeutic drugs based on diagnostic genes. (A) Molecular docking of RO 3306 with CDK1; (B) Molecular docking of AR234960 with COL4A2; (C, D) Determination of the optimal drug concentrations using the CCK-8 assay; (E–G) Western blotting analysis of MMP3 and SRGN expression levels in vitro under different treatment conditions; (H–J) Western blotting analysis of MMP3 and SRGN expression levels in animal experiments following different treatment regimens; (K, L) qPCR analysis of MMP3 and SRGN mRNA levels after different treatment conditions. Data are presented as mean ± SD, with experiments repeated three times; *P < 0.05, **P < 0.01, ***P < 0.001, **** indicates that there is a highly significant correlation between the two (one-way ANOVA with Bonferroni post hoc test).

Article Snippet: Antibodies: COL4A2 (Collagen Type IV Alpha 2 Chain, 1:1000) – Proteintech (#14695-1-AP, RRID: AB_10699877); CDK1 (Cyclin-Dependent Kinase 1, 1:1000) – Proteintech (#19532-1-AP, RRID: AB_2881388); MMP3 (Matrix Metalloproteinase 3, 1:1000) – Proteintech (#17873-1-AP, RRID: AB_2138307); SRGN (Serglycin, 1:1000) – Thermo Fisher Scientific (#PA5-113692, RRID: AB_2884207); HRP-conjugated anti-mouse IgG (H+L, 1:5000) – Proteintech (#SA00001-1, RRID: AB_2722565); HRP-conjugated anti-rabbit IgG (H+L, 1:5000) – Proteintech (#SA00001-2, RRID: AB_2722564).

Techniques: Diagnostic Assay, CCK-8 Assay, Western Blot, Expressing, In Vitro

Journal: iScience

Article Title: HucMSCs can alleviate abnormal vasculogenesis induced by high glucose through the MAPK signaling pathway

doi: 10.1016/j.isci.2024.111354

Figure Lengend Snippet:

Article Snippet: COL4A2-specific Polyclonal antibody , Proteintech , Cat# 55131-1-AP; RRID: AB_2881273.

Techniques: Recombinant, Gentle, Membrane, Lysis, TUNEL Assay, Apoptosis Assay, Fluorescence, Software